4peu

X-ray diffraction
1.8Å resolution

Structure of the polysaccharide lyase-like protein Cthe_2159 from C. thermocellum, native form with Calcium bound

Released:
Primary publication:
A new family of β-helix proteins with similarities to the polysaccharide lyases.
Acta Crystallogr D Biol Crystallogr 70 2583-92 (2014)
PMID: 25286843

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-107065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate-binding domain-containing protein Cthe_2159 Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 32.86 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DHD2 (Residues: 2-286; Coverage: 100%)
Gene name: Cthe_2159
Sequence domains: Carbohydrate-binding domain-containing protein Cthe_2159

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 70.7Å b: 123.429Å c: 34.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.146 0.171
Expression system: Escherichia coli BL21(DE3)