› 4pi7

X-ray diffraction
1.6Å resolution

Crystal structure of S. Aureus Autolysin E in complex with disaccharide NAM-NAG

Released:
DOI: 10.2210/pdb4pi7/pdb
PDB entry 4pi7 coloured by chain, front view.
PDB entry 4pi7 coloured by chain, side view.
PDB entry 4pi7 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
The mechanism behind the selection of two different cleavage sites in NAG-NAM polymers
Mihelic M, Vlahovicek-Kahlina K, Renko M, Mesnage S, Dobersek A, Taler-Vercic A, Jakas A, Turk D
IUCrJ 4 185-198 (2017)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 26.07 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JT72 (Residues: 35-258; Coverage: 87%)
Gene name: SAV2307
Sequence domains: Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: AMU, NAG
Carbohydrate polymer
2 bound ligands:
Ligand CL 11 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 46.31Å b: 69.782Å c: 73.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Catalytic diversity and cell wall binding repeats in the phage-encoded endolysins.
Broendum et al. (2018)
2 other articles