› 4psb

X-ray diffraction
1.42Å resolution

Crystal Structure of Phytohormone Binding Protein from Vigna radiata in complex with gibberellic acid (GA3)

Released:
DOI: 10.2210/pdb4psb/pdb
PDB entry 4psb coloured by chain, front view.
PDB entry 4psb coloured by chain, side view.
PDB entry 4psb coloured by chain, top view.
Source organism: Vigna radiata
Primary publication:
Specific binding of gibberellic acid by cytokinin-specific binding proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold.
Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M
Acta Crystallogr D Biol Crystallogr 70 2032-41 (2014)
PMID: 25004979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytohormone-binding protein CSBP Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.61 KDa
Source organism: Vigna radiata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1S3THR8 (Residues: 1-155; Coverage: 100%)
Gene names: CSBP, LOC106755646
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand GA3 1 x GA3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 34.38Å b: 54.29Å c: 71.09Å
α: 90° β: 98.7° γ: 90°
R-values:
R R work R free
0.154 0.152 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family.
Aglas et al. (2020)
1 more

1 mention without citation

A redox-responsive self-assembling COA-4-arm PEG prodrug nanosystem for dual drug delivery suppresses cancer metastasis and drug resistance by downregulating hsp90 expression.
Zhou et al. (2023)