› 4psx

X-ray diffraction
2.51Å resolution

Crystal structure of histone acetyltransferase complex

Released:
DOI: 10.2210/pdb4psx/pdb
PDB entry 4psx coloured by chain, front view.
PDB entry 4psx coloured by chain, side view.
PDB entry 4psx coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex.
Li Y, Zhang L, Liu T, Chai C, Fang Q, Wu H, Agudelo Garcia PA, Han Z, Zong S, Yu Y, Zhang X, Parthun MR, Chai J, Xu RM, Yang M
OpenAccess logo Genes Dev 28 1217-27 (2014)
PMID: 24835250

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-525978 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone acetyltransferase type B catalytic subunit Chains: A, D
Molecule details ›
Chains: A, D
Length: 320 amino acids
Theoretical weight: 37.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12341 (Residues: 7-319; Coverage: 84%)
Gene names: HAT1, LPA16W, YP8132.12, YPL001W
Sequence domains:
Structure domains:
Histone acetyltransferase type B subunit 2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 401 amino acids
Theoretical weight: 45.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P39984 (Residues: 8-389; Coverage: 95%)
Gene names: HAT2, YEL056W
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H4 Chains: C, F
Molecule details ›
Chains: C, F
Length: 48 amino acids
Theoretical weight: 5.09 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P02309 (Residues: 2-49; Coverage: 47%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H3 Chains: P, Y
Molecule details ›
Chains: P, Y
Length: 15 amino acids
Theoretical weight: 1.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61830 (Residues: 2-16; Coverage: 11%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 78.548Å b: 85.331Å c: 114.51Å
α: 75.97° β: 72.4° γ: 66.06°
R-values:
R R work R free
0.183 0.181 0.223
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Histone chaperone networks shaping chromatin function.
Hammond et al. (2017)
3 more

2 mentions without citation

Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex.
Li et al. (2014)
1 more