› 4q7t

X-ray diffraction
1.94Å resolution

Crystal structure of photoswitchable fluorescent protein PSmOrange

Released:
DOI: 10.2210/pdb4q7t/pdb
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PDB entry 4q7t coloured by chain, front view.
PDB entry 4q7t coloured by chain, side view.
PDB entry 4q7t coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Orange fluorescent proteins: structural studies of LSSmOrange, PSmOrange and PSmOrange2.
Pletnev S, Shcherbakova DM, Subach OM, Pletneva NV, Malashkevich VN, Almo SC, Dauter Z, Verkhusha VV
OpenAccess logo PLoS One 9 e99136 (2014)
PMID: 24960050

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 28.21 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWW2 (Residues: 1-236; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OFM 2 x OFM

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 45.363Å b: 50.683Å c: 52.414Å
α: 94.71° β: 90.03° γ: 106.59°
R-values:
R R work R free
0.203 0.2 0.266
Expression system: Escherichia coli

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Citations

7 citation in other articles

Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.
Ast et al. (2017)
6 more

1 mention without citation

Orange fluorescent proteins: structural studies of LSSmOrange, PSmOrange and PSmOrange2.
Pletnev et al. (2014)

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