› 4qc9

X-ray diffraction
2.26Å resolution

Crystal structure of Vaccinia virus uracil-DNA glycosylase mutant 3GD4

Released:
DOI: 10.2210/pdb4qc9/pdb
PDB entry 4qc9 coloured by chain, front view.
PDB entry 4qc9 coloured by chain, side view.
PDB entry 4qc9 coloured by chain, top view.
Source organism: Vaccinia virus Ankara
Entry authors: Sartmatova D, Nash T, Schormann N, Nuth M, Ricciardi R, Banerjee S, Chattopadhyay D

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 221 amino acids
Theoretical weight: 25.22 KDa
Source organism: Vaccinia virus Ankara
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04303 (Residues: 1-218; Coverage: 100%)
Gene names: D4R, OPG116, UNG, VACWR109
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 93.378Å b: 93.378Å c: 285.182Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.229
Expression system: Escherichia coli BL21(DE3)

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