› 4qf8

X-ray diffraction
1.65Å resolution

Crystal Structure of the Complex of Phospholipase A2 with Spermidine at 1.65 A Resolution

Released:
DOI: 10.2210/pdb4qf8/pdb
PDB entry 4qf8 coloured by chain, front view.
PDB entry 4qf8 coloured by chain, side view.
PDB entry 4qf8 coloured by chain, top view.
Source organism: Daboia russelii pulchella
Primary publication:
Structures and binding studies of the complexes of phospholipase A2 with five inhibitors.
Shukla PK, Gautam L, Sinha M, Kaur P, Sharma S, Singh TP
Biochim Biophys Acta 1854 269-77 (2015)
PMID: 25541253

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 VRV-PL-VIIIa Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.63 KDa
Source organism: Daboia russelii pulchella
UniProt:
  • Canonical: P59071 (Residues: 1-121; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand SPD 1 x SPD
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P43
Unit cell:
a: 51.555Å b: 51.555Å c: 47.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.237

Quick links

Citations

6 review citations

Resveratrol: How Much Wine Do You Have to Drink to Stay Healthy?
Weiskirchen et al. (2016)
5 more

1 mention without citation

Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum.
Dos Santos Junior et al. (2020)