› 4qhj

X-ray diffraction
1.75Å resolution

Crystal structure of Methanocaldococcus jannaschii selecase mutant I100F+H107F

Released:
DOI: 10.2210/pdb4qhj/pdb
PDB entry 4qhj coloured by chain, front view.
PDB entry 4qhj coloured by chain, side view.
PDB entry 4qhj coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Multiple stable conformations account for reversible concentration-dependent oligomerization and autoinhibition of a metamorphic metallopeptidase.
López-Pelegrín M, Cerdà-Costa N, Cintas-Pedrola A, Herranz-Trillo F, Bernadó P, Peinado JR, Arolas JL, Gomis-Rüth FX
Angew Chem Int Ed Engl 53 10624-30 (2014)
PMID: 25159620

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ1213 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 13.15 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58610 (Residues: 1-110; Coverage: 100%)
Gene name: MJ1213
Sequence domains: BlaR1 peptidase M56

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 5 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 49.39Å b: 49.84Å c: 104.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural metamorphism and polymorphism in proteins on the brink of thermodynamic stability.
Kulkarni et al. (2018)
5 more