› 4qjh

X-ray diffraction
3.88Å resolution

Crystal Structure of the Twister Ribozyme with the Nucleotide 5'- to the Cleavage Site Ordered at 4.1 A Resolution

Released:
DOI: 10.2210/pdb4qjh/pdb
PDB entry 4qjh coloured by chain, front view.
PDB entry 4qjh coloured by chain, side view.
PDB entry 4qjh coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme.
Eiler D, Wang J, Steitz TA
Proc Natl Acad Sci U S A 111 13028-33 (2014)
PMID: 25157168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196862 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
Twister Ribozyme Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 26 nucleotides
Theoretical weight: 8.26 KDa
Source organism: Synthetic construct
Expression system: Not provided
Twister Ribozyme Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 48 nucleotides
Theoretical weight: 15.72 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
1 modified residue:
Ligand GTP 3 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6122
Unit cell:
a: 228.77Å b: 228.77Å c: 101.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.232
Expression system: Not provided

Quick links

Citations

7 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
6 more

12 mentions without citation

Chemistry and Biology of Self-Cleaving Ribozymes.
Jimenez et al. (2015)
11 more