› 4qr1

X-ray diffraction
2.19Å resolution

Crystal structure of Streptococcus pyogenes Cas2 at pH 6.5

Released:
DOI: 10.2210/pdb4qr1/pdb
PDB entry 4qr1 coloured by chain, front view.
PDB entry 4qr1 coloured by chain, side view.
PDB entry 4qr1 coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Structural and functional characterization of Streptococcus pyogenes Cas2 protein under different pH conditions.
Ka D, Kim D, Baek G, Bae E
Biochem Biophys Res Commun 451 152-7 (2014)
PMID: 25079131

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.26 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99YS8 (Residues: 1-97; Coverage: 100%)
Gene names: SPy_1561, cas2
Sequence domains: CRISPR associated protein Cas2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P41212
Unit cell:
a: 55.054Å b: 55.054Å c: 117.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.267
Expression system: Escherichia coli

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Citations

7 review citations

Evolutionary Genomics of Defense Systems in Archaea and Bacteria.
Koonin et al. (2017)
6 more