› 4qri

X-ray diffraction
2.35Å resolution

2.35 Angstrom resolution crystal structure of hypoxanthine-guanine-xanthine phosphoribosyltransferase from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Released:
DOI: 10.2210/pdb4qri/pdb
PDB entry 4qri coloured by chain, front view.
PDB entry 4qri coloured by chain, side view.
PDB entry 4qri coloured by chain, top view.
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Entry authors: Halavaty AS, Minasov G, Dubrovska I, Flores KJ, Shuvalova L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.95 KDa
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72LV5 (Residues: 1-183; Coverage: 100%)
Gene names: LIC_13438, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 74.647Å b: 74.647Å c: 162.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli BL21(DE3)

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