› 4r33

X-ray diffraction
1.78Å resolution

X-ray structure of the tryptophan lyase NosL with Tryptophan and S-adenosyl-L-homocysteine bound

Released:
DOI: 10.2210/pdb4r33/pdb
PDB entry 4r33 coloured by chain, front view.
PDB entry 4r33 coloured by chain, side view.
PDB entry 4r33 coloured by chain, top view.
Source organism: Streptomyces actuosus
Primary publication:
Crystal structure of tryptophan lyase (NosL): evidence for radical formation at the amino group of tryptophan.
Nicolet Y, Zeppieri L, Amara P, Fontecilla-Camps JC
Angew Chem Int Ed Engl 53 11840-4 (2014)
PMID: 25196319

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-methyl-2-indolic acid synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 46.83 KDa
Source organism: Streptomyces actuosus
Expression system: Escherichia coli
UniProt:
  • Canonical: C6FX51 (Residues: 1-400; Coverage: 100%)
Gene names: DMT42_23170, nocL, nosL
Structure domains: Aldolase class I

Ligands and Environments

7 bound ligands:
Ligand SAH 2 x SAH
Ligand SF4 2 x SF4
Ligand TRP 2 x TRP
Ligand GOL 4 x GOL
Ligand K 2 x K
Ligand NA 1 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P2
Unit cell:
a: 94.72Å b: 47.23Å c: 114.36Å
α: 90° β: 108.71° γ: 90°
R-values:
R R work R free
0.16 0.159 0.181
Expression system: Escherichia coli

Quick links

Citations

10 review citations

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
9 more

4 mentions without citation

New Insights into the Biosynthetic Logic of Ribosomally Synthesized and Post-translationally Modified Peptide Natural Products.
Ortega et al. (2016)
3 more