› 4r5q

X-ray diffraction
2.65Å resolution

Crystal structure and nuclease activity of the CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis containing a [2Fe-2S] cluster

Released:
DOI: 10.2210/pdb4r5q/pdb
PDB entry 4r5q coloured by chain, front view.
PDB entry 4r5q coloured by chain, side view.
PDB entry 4r5q coloured by chain, top view.
Source organism: Pyrobaculum calidifontis JCM 11548
Entry authors: Nocek B, Skarina T, Lemak S, Brown G, Savchenko A, Joachimiak A, Yakunin A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated exonuclease Cas4 Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 25.03 KDa
Source organism: Pyrobaculum calidifontis JCM 11548
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3MTK6 (Residues: 1-213; Coverage: 100%)
Gene names: Pcal_0546, cas4
Sequence domains: Domain of unknown function DUF83
Structure domains: Lambda Exonuclease; Chain A

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I422
Unit cell:
a: 117.836Å b: 117.836Å c: 89.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.253
Expression system: Escherichia coli BL21(DE3)

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