› 4r6w

X-ray diffraction
1.59Å resolution

Plasmodium falciparum phosphoethanolamine methyltransferase D128A mutant in complex with S-adenosylhomocysteine and phosphocholine

Released:
DOI: 10.2210/pdb4r6w/pdb
PDB entry 4r6w coloured by chain, front view.
PDB entry 4r6w coloured by chain, side view.
PDB entry 4r6w coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
An alternative mechanism for the methylation of phosphoethanolamine catalyzed by Plasmodium falciparum phosphoethanolamine methyltransferase.
Saen-Oon S, Lee SG, Jez JM, Guallar V
J Biol Chem 289 33815-25 (2014)
PMID: 25288796

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoethanolamine N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 30.11 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IDQ9 (Residues: 9-266; Coverage: 97%)
Gene names: PF3D7_1343000, PMT
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand PC 2 x PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P2
Unit cell:
a: 77.171Å b: 44.062Å c: 89.147Å
α: 90° β: 108.09° γ: 90°
R-values:
R R work R free
0.161 0.159 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

High-resolution metabolomics to discover potential parasite-specific biomarkers in a Plasmodium falciparum erythrocytic stage culture system.
Park et al. (2015)
6 more

1 mention without citation

An alternative mechanism for the methylation of phosphoethanolamine catalyzed by Plasmodium falciparum phosphoethanolamine methyltransferase.
Saen-Oon et al. (2014)