› 4rbp

X-ray diffraction
1.85Å resolution

Crystal structure of HIV neutralizing antibody 2G12 in complex with a bacterial oligosaccharide analog of mammalian oligomanose

Released:
DOI: 10.2210/pdb4rbp/pdb
PDB entry 4rbp coloured by chain, front view.
PDB entry 4rbp coloured by chain, side view.
PDB entry 4rbp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the HIV neutralizing antibody 2G12 in complex with a bacterial oligosaccharide analog of mammalian oligomannose.
Stanfield RL, De Castro C, Marzaioli AM, Wilson IA, Pantophlet R
Glycobiology 25 412-9 (2015)
PMID: 25380763

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213611 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 2G12 light chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 213 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Fab 2G12 heavy chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 224 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: KDO, MAN
Carbohydrate polymer
1 bound ligand:
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 45.3Å b: 130.88Å c: 170.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.252

Quick links

Citations

7 review citations

Antibody responses to viral infections: a structural perspective across three different enveloped viruses.
Murin et al. (2019)
6 more

2 mentions without citation

Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity.
Pantophlet et al. (2017)
1 more