› 4rep

X-ray diffraction
1.97Å resolution

Crystal Structure of gamma-carotenoid desaturase

Released:
DOI: 10.2210/pdb4rep/pdb
PDB entry 4rep coloured by chain, front view.
PDB entry 4rep coloured by chain, side view.
PDB entry 4rep coloured by chain, top view.
Source organism: Nonlabens dokdonensis DSW-6
Primary publication:
Crystal structure of 1'-OH-carotenoid 3,4-desaturase from Nonlabens dokdonensis DSW-6.
Ahn JW, Kim KJ
Enzyme Microb Technol 77 29-37 (2015)
PMID: 26138397

Function and Biology Details

Reaction catalysed: 
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-hydroxycarotenoid 3,4-desaturase Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 55.91 KDa
Source organism: Nonlabens dokdonensis DSW-6
Expression system: Escherichia coli
UniProt:
  • Canonical: L7WC64 (Residues: 1-487; Coverage: 100%)
Gene names: DDD_2394, crtD
Sequence domains: Flavin containing amine oxidoreductase

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1), SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 48.369Å b: 72.835Å c: 68.137Å
α: 90° β: 110.29° γ: 90°
R-values:
R R work R free
0.163 0.16 0.224
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Structure of Phytoene Desaturase Provides Insights into Herbicide Binding and Reaction Mechanisms Involved in Carotene Desaturation.
Brausemann et al. (2017)
2 more

6 mentions without citation

Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.
Altman et al. (2004)
5 more