› 4rk5

X-ray diffraction
1.35Å resolution

Crystal structure of LacI family transcriptional regulator from Lactobacillus casei, Target EFI-512911, with bound sucrose

Released:
DOI: 10.2210/pdb4rk5/pdb
PDB entry 4rk5 coloured by chain, front view.
PDB entry 4rk5 coloured by chain, side view.
PDB entry 4rk5 coloured by chain, top view.
Source organism: Lacticaseibacillus paracasei ATCC 334
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Chamala S, Attonito JD, Scott Glenn A, Chowdhury S, Lafleur J, Siedel RD, Hillerich B, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
HTH lacI-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.91 KDa
Source organism: Lacticaseibacillus paracasei ATCC 334
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q036L9 (Residues: 54-333; Coverage: 84%)
Gene name: LSEI_2103
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
2 bound ligands:
Ligand NA 1 x NA
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R32
Unit cell:
a: 108.754Å b: 108.754Å c: 125.763Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.11 0.109 0.141
Expression system: Escherichia coli BL21(DE3)

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