4rwf

X-ray diffraction
1.76Å resolution

Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound adrenomedullin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-130166 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Calcitonin gene-related peptide type 1 receptor; Maltose/maltodextrin-binding periplasmic protein; Receptor activity-modifying protein 2 Chain: A
Molecule details ›
Chain: A
Length: 591 amino acids
Theoretical weight: 66.31 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 26-392; Coverage: 99%)
  • Canonical: O60895 (Residues: 49-138; Coverage: 68%)
  • Canonical: Q16602 (Residues: 31-144; Coverage: 26%)
Gene names: CALCRL, CGRPR, JW3994, RAMP2, b4034, malE
Sequence domains:
Adrenomedullin Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 3.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P35318 (Residues: 119-146; Coverage: 17%)
Gene names: ADM, AM

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 71.454Å b: 84.283Å c: 115.764Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.2
Expression systems:
  • Escherichia coli
  • Not provided