› 4s2o

X-ray diffraction
1.7Å resolution

OXA-10 in complex with Avibactam

Released:
DOI: 10.2210/pdb4s2o/pdb
Model geometry
Fit model/data
PDB entry 4s2o coloured by chain, front view.
PDB entry 4s2o coloured by chain, side view.
PDB entry 4s2o coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Molecular Mechanism of Avibactam-Mediated β-Lactamase Inhibition.
King DT, King AM, Lal SM, Wright GD, Strynadka NC
ACS Infect Dis 1 175-84 (2015)
PMID: 27622530

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14489 (Residues: 20-265; Coverage: 100%)
Gene names: OXA-10, bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand NXL 2 x NXL
Ligand CO 4 x CO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 48.6Å b: 96.5Å c: 125.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.23
Expression system: Escherichia coli

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Citations

7 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
6 more

2 mentions without citation

Structural insights into the enhanced carbapenemase efficiency of OXA-655 compared to OXA-10.
Leiros et al. (2020)
1 more