› 4tqv

X-ray diffraction
4.5Å resolution

Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate

Released:
DOI: 10.2210/pdb4tqv/pdb
PDB entry 4tqv coloured by chain, front view.
PDB entry 4tqv coloured by chain, side view.
PDB entry 4tqv coloured by chain, top view.
Source organism: Sphingomonas sp.
Primary publication:
Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate.
Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K
Structure 23 1643-1654 (2015)
PMID: 26235029

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575900 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ABC transmembrane type-1 domain-containing protein Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 301 amino acids
Theoretical weight: 34.29 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT8 (Residues: 25-324; Coverage: 93%)
Gene name: algM1
Sequence domains: Binding-protein-dependent transport system inner membrane component
ABC transmembrane type-1 domain-containing protein Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 305 amino acids
Theoretical weight: 34.5 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT7 (Residues: 1-293; Coverage: 100%)
Gene name: algM2
Sequence domains: Binding-protein-dependent transport system inner membrane component
ABC transporter domain-containing protein Chains: C, D, G, H, K, L, O, P
Molecule details ›
Chains: C, D, G, H, K, L, O, P
Length: 363 amino acids
Theoretical weight: 39.57 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT9 (Residues: 1-363; Coverage: 100%)
Gene name: algS
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P1
Unit cell:
a: 115.245Å b: 151.187Å c: 162.406Å
α: 68.66° β: 81.76° γ: 90.1°
R-values:
R R work R free
0.28 0.279 0.32
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
4 more