› 4ttr

X-ray diffraction
2.1Å resolution

Crystal structure of Legionella pneumophila dephospho-CoA kinase in complex with Bu2

Released:
DOI: 10.2210/pdb4ttr/pdb
PDB entry 4ttr coloured by chain, front view.
PDB entry 4ttr coloured by chain, side view.
PDB entry 4ttr coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Crystal structure of Legionella pneumophila dephospho-CoA kinase reveals a non-canonical conformation of P-loop.
Gong X, Chen X, Yu D, Zhang N, Zhu Z, Niu L, Mao Y, Ge H
J Struct Biol 188 233-9 (2014)
PMID: 25449315

Function and Biology Details

Reaction catalysed: 
ATP + 3'-dephospho-CoA = ADP + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dephospho-CoA kinase Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.63 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZVH3 (Residues: 1-201; Coverage: 100%)
Gene names: coaE, lpg1467
Sequence domains: Dephospho-CoA kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand BU2 1 x BU2
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 36.29Å b: 82.2Å c: 81.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.228
Expression system: Escherichia coli

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Citations

1 mention without citation

Crystal structure of Legionella pneumophila dephospho-CoA kinase reveals a non-canonical conformation of P-loop.
Gong et al. (2014)