› 4twb

X-ray diffraction
2.8Å resolution

Sulfolobus solfataricus ribose-phosphate pyrophosphokinase

Released:
DOI: 10.2210/pdb4twb/pdb
PDB entry 4twb coloured by chain, front view.
PDB entry 4twb coloured by chain, side view.
PDB entry 4twb coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure of dimeric, recombinant Sulfolobus solfataricus phosphoribosyl diphosphate synthase: a bent dimer defining the adenine specificity of the substrate ATP.
Andersen RW, Leggio LL, Hove-Jensen B, Kadziola A
Extremophiles 19 407-15 (2015)
PMID: 25605536

Function and Biology Details

Reaction catalysed: 
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-phosphate pyrophosphokinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 291 amino acids
Theoretical weight: 32.22 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z86 (Residues: 1-291; Coverage: 100%)
Gene names: SSO1045, prs
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand AMP 6 x AMP
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 80.217Å b: 92.846Å c: 138.338Å
α: 90° β: 90.91° γ: 90°
R-values:
R R work R free
0.236 0.235 0.265
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Pcal_1127, a highly stable and efficient ribose-5-phosphate pyrophosphokinase from Pyrobaculum calidifontis.
Bibi et al. (2016)
1 more

1 mention without citation

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)