› 4txa

X-ray diffraction
2.75Å resolution

Crystal structure of N-terminus of Roquin

Released:
DOI: 10.2210/pdb4txa/pdb
PDB entry 4txa coloured by chain, front view.
PDB entry 4txa coloured by chain, side view.
PDB entry 4txa coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Kershaw NJ, Vinuesa CG, Babon JJ

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roquin-1 Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 54.25 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4VGL6 (Residues: 1-484; Coverage: 43%)
Gene names: Gm551, Kiaa2025, Rc3h1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 142.804Å b: 80.191Å c: 54.858Å
α: 90° β: 111.06° γ: 90°
R-values:
R R work R free
0.22 0.218 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links