› 4u2t

X-ray diffraction
1.22Å resolution

Cholesterol oxidase in the oxidised state complexed with isopropanol

Released:
DOI: 10.2210/pdb4u2t/pdb
PDB entry 4u2t coloured by chain, front view.
PDB entry 4u2t coloured by chain, side view.
PDB entry 4u2t coloured by chain, top view.
Source organism: Streptomyces sp. SA-COO
Primary publication:
High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization.
Golden E, Karton A, Vrielink A
Acta Crystallogr D Biol Crystallogr 70 3155-66 (2014)
PMID: 25478834

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.8 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 51.4Å b: 73.97Å c: 63.59Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.12 0.119 0.149
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

The Nonphysiological Reductant Sodium Dithionite and [FeFe] Hydrogenase: Influence on the Enzyme Mechanism.
Martini et al. (2021)
1 more

2 mentions without citation

An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase.
Golden et al. (2017)
1 more