› 4ubg

X-ray diffraction
1.9Å resolution

Resting state of rat cysteine dioxygenase C93G variant

Released:
DOI: 10.2210/pdb4ubg/pdb
PDB entry 4ubg coloured by chain, front view.
PDB entry 4ubg coloured by chain, side view.
PDB entry 4ubg coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The Cys-Tyr cross-link of cysteine dioxygenase changes the optimal pH of the reaction without a structural change.
Davies CG, Fellner M, Tchesnokov EP, Wilbanks SM, Jameson GN
Biochemistry 53 7961-8 (2014)
PMID: 25390690

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 24.21 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand NA 9 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 57.84Å b: 57.84Å c: 122.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Carbon-fluorine bond cleavage mediated by metalloenzymes.
Wang et al. (2020)
2 more

1 mention without citation

Structure-Based Insights into the Role of the Cys-Tyr Crosslink and Inhibitor Recognition by Mammalian Cysteine Dioxygenase.
Driggers et al. (2016)