› 4ud4

X-ray diffraction
1.74Å resolution

Structural Plasticity of Cid1 Provides a Basis for its RNA Terminal Uridylyl Transferase Activity

Released:
DOI: 10.2210/pdb4ud4/pdb
PDB entry 4ud4 coloured by chain, front view.
PDB entry 4ud4 coloured by chain, side view.
PDB entry 4ud4 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structural plasticity of Cid1 provides a basis for its distributive RNA terminal uridylyl transferase activity.
Yates LA, Durrant BP, Fleurdépine S, Harlos K, Norbury CJ, Gilbert RJ
OpenAccess logo Nucleic Acids Res 43 2968-79 (2015)
PMID: 25712096

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 41.66 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13833 (Residues: 40-405; Coverage: 90%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 58.96Å b: 62.29Å c: 65.5Å
α: 76.34° β: 81.08° γ: 63.16°
R-values:
R R work R free
0.174 0.172 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

RNA uridylation: a key posttranscriptional modification shaping the coding and noncoding transcriptome.
De Almeida et al. (2018)
3 more

1 mention without citation

Structural plasticity of Cid1 provides a basis for its distributive RNA terminal uridylyl transferase activity.
Yates et al. (2015)