› 4uii

X-ray diffraction
2.83Å resolution

Crystal structure of the Azotobacter vinelandii globin-coupled oxygen sensor in the aquo-met form

Released:
DOI: 10.2210/pdb4uii/pdb
PDB entry 4uii coloured by chain, front view.
PDB entry 4uii coloured by chain, side view.
PDB entry 4uii coloured by chain, top view.
Source organism: Azotobacter vinelandii
Entry authors: Germani F, De Schutter A, Pesce A, Berghmans H, Van Hauwaert M-L, Cuypers B, Bruno S, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Nardini M, Dewilde S

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase DosC Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.84 KDa
Source organism: Azotobacter vinelandii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M9YE33 (Residues: 1-170; Coverage: 37%)
Gene name: AvCA6_00420
Sequence domains: Protoglobin

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 47.89Å b: 49.49Å c: 62.77Å
α: 90° β: 91.81° γ: 90°
R-values:
R R work R free
0.254 0.252 0.297
Expression system: Escherichia coli BL21(DE3)

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