› 4umz

X-ray diffraction
2.32Å resolution

PikC D50N mutant in complex with the engineered substrate mimic bearing a 2-dimethylaminomethylbenzoate group

Released:
DOI: 10.2210/pdb4umz/pdb
PDB entry 4umz coloured by chain, front view.
PDB entry 4umz coloured by chain, side view.
PDB entry 4umz coloured by chain, top view.
Source organism: Streptomyces venezuelae
Entry authors: Podust LM, Vieira DF

Function and Biology Details

Reaction catalysed: 
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase PikC Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 48.27 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O87605 (Residues: 1-416; Coverage: 100%)
Gene names: picK, pikC
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand XJO 2 x XJO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 59.061Å b: 110.703Å c: 151.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.245
Expression system: Escherichia coli K-12

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