› 4un1

X-ray diffraction
1.97Å resolution

Sirohaem decarboxylase AhbA/B - an enzyme with structural homology to the Lrp/AsnC transcription factor family that is part of the alternative haem biosynthesis pathway.

Released:
DOI: 10.2210/pdb4un1/pdb
PDB entry 4un1 coloured by chain, front view.
PDB entry 4un1 coloured by chain, side view.
PDB entry 4un1 coloured by chain, top view.
Source organism: Desulfovibrio desulfuricans
Primary publication:
The structure, function and properties of sirohaem decarboxylase--an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway.
Palmer DJ, Schroeder S, Lawrence AD, Deery E, Lobo SA, Saraiva LM, McLean KJ, Munro AW, Ferguson SJ, Pickersgill RW, Brown DG, Warren MJ
OpenAccess logo Mol Microbiol 93 247-61 (2014)
PMID: 24865947

Function and Biology Details

Reaction catalysed: 
Siroheme = 12,18-didecarboxysiroheme + 2 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110734 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Siroheme decarboxylase alpha subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 173 amino acids
Theoretical weight: 19.21 KDa
Source organism: Desulfovibrio desulfuricans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8J364 (Residues: 1-173; Coverage: 100%)
Gene name: Ddes_0286
Sequence domains:
Siroheme decarboxylase beta subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 179 amino acids
Theoretical weight: 20.11 KDa
Source organism: Desulfovibrio desulfuricans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8J3A4 (Residues: 1-159; Coverage: 100%)
Gene name: Ddes_2018
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand OBV 2 x OBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 55.3Å b: 78.6Å c: 150.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
1 more

2 mentions without citation

Evolutionary relationships between heme-binding ferredoxin α + β barrels.
Acharya et al. (2016)
1 more