› 4uoy

X-ray diffraction
2.3Å resolution

Crystal structure of YgjG in complex with Pyridoxal-5'-phosphate

Released:
DOI: 10.2210/pdb4uoy/pdb
PDB entry 4uoy coloured by chain, front view.
PDB entry 4uoy coloured by chain, side view.
PDB entry 4uoy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of putrescine aminotransferase from Escherichia coli provides insights into the substrate specificity among class III aminotransferases.
Cha HJ, Jeong JH, Rojviriya C, Kim YG
OpenAccess logo PLoS One 9 e113212 (2014)
PMID: 25423189

Function and Biology Details

Reactions catalysed: 
An alpha,omega-diamine + 2-oxoglutarate = an omega-aminoaldehyde + L-glutamate
Putrescine + 2-oxoglutarate = L-glutamate + 4-aminobutanal + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 467 amino acids
Theoretical weight: 50.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P42588 (Residues: 1-459; Coverage: 100%)
Gene names: JW5510, b3073, patA, ygjG
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand FMT 1 x FMT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 120.945Å b: 129.319Å c: 131.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
Steffen-Munsberg et al. (2015)
2 more

1 mention without citation

Structure of putrescine aminotransferase from Escherichia coli provides insights into the substrate specificity among class III aminotransferases.
Cha et al. (2014)