› 4uq8

Electron Microscopy
4.95Å resolution

Electron cryo-microscopy of bovine Complex I

Released:
DOI: 10.2210/pdb4uq8/pdb
PDB entry 4uq8 coloured by chain, front view.
PDB entry 4uq8 coloured by chain, side view.
PDB entry 4uq8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Architecture of mammalian respiratory complex I.
Vinothkumar KR, Zhu J, Hirst J
OpenAccess logo Nature 515 80-84 (2014)
PMID: 25209663
Related structures: EMD-2676

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 49-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165234 (preferred)
Entry contents:
43 distinct polypeptide molecules
Macromolecules (43 distinct):
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 3 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 9.46 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 12.26 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL Chain: C
Molecule details ›
Chain: C
Length: 154 amino acids
Theoretical weight: 13.12 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL Chain: D
Molecule details ›
Chain: D
Length: 384 amino acids
Theoretical weight: 32.7 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL Chain: E
Molecule details ›
Chain: E
Length: 159 amino acids
Theoretical weight: 13.62 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL Chain: F
Molecule details ›
Chain: F
Length: 411 amino acids
Theoretical weight: 35.07 KDa
Source organism: Bos taurus
NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL Chain: G
Molecule details ›
Chain: G
Length: 538 amino acids
Theoretical weight: 46.06 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 1 Chain: H
Molecule details ›
Chain: H
Length: 313 amino acids
Theoretical weight: 26.66 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL Chain: I
Molecule details ›
Chain: I
Length: 162 amino acids
Theoretical weight: 13.95 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 6 Chain: J
Molecule details ›
Chain: J
Length: 171 amino acids
Theoretical weight: 14.57 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4L Chain: K
Molecule details ›
Chain: K
Length: 84 amino acids
Theoretical weight: 7.17 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 5 Chain: L
Molecule details ›
Chain: L
Length: 601 amino acids
Theoretical weight: 51.17 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4 Chain: M
Molecule details ›
Chain: M
Length: 453 amino acids
Theoretical weight: 38.57 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE CHAIN 2 Chain: N
Molecule details ›
Chain: N
Length: 345 amino acids
Theoretical weight: 29.38 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 10 Chain: O
Molecule details ›
Chain: O
Length: 220 amino acids
Theoretical weight: 18.74 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL Chain: P
Molecule details ›
Chain: P
Length: 303 amino acids
Theoretical weight: 25.8 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 4 Chain: Q
Molecule details ›
Chain: Q
Length: 85 amino acids
Theoretical weight: 7.25 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] SUBUNIT 6 Chain: R
Molecule details ›
Chain: R
Length: 47 amino acids
Theoretical weight: 4.02 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2 Chain: S
Molecule details ›
Chain: S
Length: 80 amino acids
Theoretical weight: 6.83 KDa
Source organism: Bos taurus
ACYL CARRIER PROTEIN, MITOCHONDRIAL Chain: T
Molecule details ›
Chain: T
Length: 75 amino acids
Theoretical weight: 6.4 KDa
Source organism: Bos taurus
NADH UBIQUINONE DEHYDROGENASE Chain: U
Molecule details ›
Chain: U
Length: 79 amino acids
Theoretical weight: 6.73 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 5 Chain: V
Molecule details ›
Chain: V
Length: 71 amino acids
Theoretical weight: 6.06 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 6 Chain: W
Molecule details ›
Chain: W
Length: 72 amino acids
Theoretical weight: 6.15 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8 Chain: X
Molecule details ›
Chain: X
Length: 79 amino acids
Theoretical weight: 6.74 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT; 11 Chain: Y
Molecule details ›
Chain: Y
Length: 106 amino acids
Theoretical weight: 9.04 KDa
Source organism: Bos taurus
NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13 Chain: Z
Molecule details ›
Chain: Z
Length: 65 amino acids
Theoretical weight: 5.55 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: a
Molecule details ›
Chain: a
Length: 29 amino acids
Theoretical weight: 2.49 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: b
Molecule details ›
Chain: b
Length: 42 amino acids
Theoretical weight: 3.59 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chains: c, w
Molecule details ›
Chains: c, w
Length: 27 amino acids
Theoretical weight: 2.32 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: d
Molecule details ›
Chain: d
Length: 39 amino acids
Theoretical weight: 3.34 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: e
Molecule details ›
Chain: e
Length: 20 amino acids
Theoretical weight: 1.72 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chains: f, h, i
Molecule details ›
Chains: f, h, i
Length: 30 amino acids
Theoretical weight: 2.57 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: g
Molecule details ›
Chain: g
Length: 22 amino acids
Theoretical weight: 1.89 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chains: j, l
Molecule details ›
Chains: j, l
Length: 24 amino acids
Theoretical weight: 2.06 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chains: k, p, s
Molecule details ›
Chains: k, p, s
Length: 28 amino acids
Theoretical weight: 2.4 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: m
Molecule details ›
Chain: m
Length: 34 amino acids
Theoretical weight: 2.91 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: n
Molecule details ›
Chain: n
Length: 59 amino acids
Theoretical weight: 5.04 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: o
Molecule details ›
Chain: o
Length: 21 amino acids
Theoretical weight: 1.81 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: q
Molecule details ›
Chain: q
Length: 25 amino acids
Theoretical weight: 2.15 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: r
Molecule details ›
Chain: r
Length: 26 amino acids
Theoretical weight: 2.23 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: t
Molecule details ›
Chain: t
Length: 57 amino acids
Theoretical weight: 4.87 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: u
Molecule details ›
Chain: u
Length: 43 amino acids
Theoretical weight: 3.68 KDa
Source organism: Bos taurus
NADH UBIQUINONE OXIDOREDUCTASE Chain: v
Molecule details ›
Chain: v
Length: 32 amino acids
Theoretical weight: 2.74 KDa
Source organism: Bos taurus

Ligands and Environments

2 bound ligands:
Ligand SF4 6 x SF4
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.95Å
Relevant EMDB volumes: EMD-2676

Quick links

Citations

73 review citations

A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury.
Chouchani et al. (2016)
72 more

14 mentions without citation

Structure and mechanism of mitochondrial electron transport chain.
Guo et al. (2018)
13 more