4ure

X-ray diffraction
1.4Å resolution

Molecular Genetic and Crystal Structural Analysis of 1-(4- Hydroxyphenyl)-Ethanol Dehydrogenase from Aromatoleum aromaticum EbN1

Released:

Function and Biology Details

Reaction catalysed:
(S)-1-phenylethanol + NAD(+) = acetophenone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-561972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclohexanol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.23 KDa
Source organism: Aromatoleum aromaticum EbN1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5P8S7 (Residues: 1-248; Coverage: 100%)
Gene names: chnA, ebA307
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 121.5Å b: 55.1Å c: 86.3Å
α: 90° β: 134.4° γ: 90°
R-values:
R R work R free
0.136 0.132 0.202
Expression system: Escherichia coli