› 4usv

X-ray diffraction
2Å resolution

Crystal structure of human soluble Adenylyl Cyclase with pyrophosphate resulting from soaking with ATP and Calcium

Released:
DOI: 10.2210/pdb4usv/pdb
PDB entry 4usv coloured by chain, front view.
PDB entry 4usv coloured by chain, side view.
PDB entry 4usv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes-implications for cyclase catalysis and evolution.
Kleinboelting S, van den Heuvel J, Steegborn C
FEBS J 281 4151-64 (2014)
PMID: 25040695

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate cyclase type 10 Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 54.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96PN6 (Residues: 1-469; Coverage: 29%)
Gene names: ADCY10, SAC
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

6 bound ligands:
Ligand CL 1 x CL
Ligand CA 1 x CA
Ligand EDO 2 x EDO
Ligand ACT 1 x ACT
Ligand POP 1 x POP
Ligand GOL 1 x GOL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P63
Unit cell:
a: 100.87Å b: 100.87Å c: 97.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.209
Expression system: Trichoplusia ni

Quick links

Citations

3 review citations

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases.
Dessauer et al. (2017)
2 more

2 mentions without citation

Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes-implications for cyclase catalysis and evolution.
Kleinboelting et al. (2014)
1 more