› 4v20

X-ray diffraction
1.5Å resolution

The 3-D structure of the cellobiohydrolase, Cel7A, from Aspergillus fumigatus, disaccharide complex

Released:
DOI: 10.2210/pdb4v20/pdb
PDB entry 4v20 coloured by chain, front view.
PDB entry 4v20 coloured by chain, side view.
PDB entry 4v20 coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution.
Moroz OV, Maranta M, Shaghasi T, Harris PV, Wilson KS, Davies GJ
Acta Crystallogr F Struct Biol Commun 71 114-20 (2015)
PMID: 25615982

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Probable 1,4-beta-D-glucan cellobiohydrolase B Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 47.14 KDa
Source organism: Aspergillus fumigatus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q4WM08 (Residues: 27-466; Coverage: 87%)
Gene names: AFUA_6G11610, cbhB
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

Carbohydrate polymer : NEW Components: SHG, BGC
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 2 x ZN
Ligand ACT 2 x ACT
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 79.56Å b: 130.78Å c: 46.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.158
Expression system: Aspergillus oryzae

Quick links

Citations

2 review citations

Cellulases from Thermophiles Found by Metagenomics.
Escuder-Rodríguez et al. (2018)
1 more

3 mentions without citation

The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution.
Moroz et al. (2015)
2 more