› 4v3a

Electron Microscopy
15Å resolution

Membrane bound pleurotolysin prepore (TMH1 lock) trapped with engineered disulphide cross-link

Released:
DOI: 10.2210/pdb4v3a/pdb
PDB entry 4v3a coloured by chain, ensemble of 20 models, front view.
PDB entry 4v3a coloured by chain, ensemble of 20 models, side view.
PDB entry 4v3a coloured by chain, ensemble of 20 models, top view.
Source organism: Pleurotus ostreatus
Primary publication:
Conformational changes during pore formation by the perforin-related protein pleurotolysin.
Lukoyanova N, Kondos SC, Farabella I, Law RH, Reboul CF, Caradoc-Davies TT, Spicer BA, Kleifeld O, Traore DA, Ekkel SM, Voskoboinik I, Trapani JA, Hatfaludi T, Oliver K, Hotze EM, Tweten RK, Whisstock JC, Topf M, Saibil HR, Dunstone MA
OpenAccess logo PLoS Biol 13 e1002049 (2015)
PMID: 25654333
Related structures: EMD-2794

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178470 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pleurotolysin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.85 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X1M9 (Residues: 2-136; Coverage: 98%)
Gene names: PlyA, plyA
Sequence domains: Aegerolysin
MACPF domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 388 amino acids
Theoretical weight: 42.91 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5W9E8 (Residues: 49-523; Coverage: 74%)
Gene name: plyB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 15Å
Relevant EMDB volumes: EMD-2794
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
26 more