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X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli.

Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH
Proc Natl Acad Sci U S A 100 8682-7 (2003)
Cited: 71 times
EuropePMC logo PMID: 12853578

Abstract

Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.

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  1. Structural insights into translational fidelity. Ogle JM, Ramakrishnan V. Annu Rev Biochem 74 129-177 (2005)
  2. Initiation of protein synthesis in bacteria. Laursen BS, Sørensen HP, Mortensen KK, Sperling-Petersen HU. Microbiol Mol Biol Rev 69 101-123 (2005)
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  5. Codon-biased translation can be regulated by wobble-base tRNA modification systems during cellular stress responses. Endres L, Dedon PC, Begley TJ. RNA Biol 12 603-614 (2015)
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  7. Toward an understanding of the structural basis of translation. Frank J. Genome Biol 4 237 (2003)
  8. The PDB and the ribosome. Moore PB. J Biol Chem 296 100561 (2021)

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