4v4n

Electron Microscopy
9Å resolution

Structure of the Methanococcus jannaschii ribosome-SecYEBeta channel complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 69-mer (preferred)
PDBe Complex ID:
PDB-CPX-158007 (preferred)
Entry contents:
63 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (67 distinct):
Protein translocase subunit SecE Chain: A7
Molecule details ›
Chain: A7
Length: 67 amino acids
Theoretical weight: 7.7 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57817 (Residues: 1-67; Coverage: 91%)
Gene names: MJ0371, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Preprotein translocase subunit SecG Chain: A8
Molecule details ›
Chain: A8
Length: 52 amino acids
Theoretical weight: 5.85 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60460 (Residues: 1-52; Coverage: 98%)
Gene names: MJ0912.1, secG
Sequence domains: Sec61beta family
50S ribosomal protein L39E Chain: Af
50S ribosomal protein L19E Chain: AQ
50S ribosomal protein L22P Chain: AS
50S ribosomal protein L23P Chain: AT
50S ribosomal protein L24P Chain: AU
50S ribosomal protein L29P Chain: AW
Protein translocase subunit SecY Chain: AX
30S ribosomal protein L7AE Chains: AG, B3
30S ribosomal protein S3AE Chain: BA
30S ribosomal protein S2P Chain: BB
30S ribosomal protein S3P Chain: BC
30S ribosomal protein S4P Chain: BD
30S ribosomal protein S4E Chain: BE
30S ribosomal protein S5P Chain: BF
30S ribosomal protein S6E Chain: BG
30S ribosomal protein S7P Chain: BH
30S ribosomal protein S8P Chain: BI
30S ribosomal protein S8E Chain: BJ
30S ribosomal protein S9P Chain: BK
30S ribosomal protein S10P Chain: BL
30S ribosomal protein S11P Chain: BM
30S ribosomal protein S12P Chain: BN
30S ribosomal protein S13P Chain: BO
30S ribosomal protein S14P Chain: BP
30S ribosomal protein S15P/S13E Chain: BQ
30S ribosomal protein S17P Chain: BR
30S ribosomal protein S17E Chain: BS
30S ribosomal protein S19P Chain: BT
30S ribosomal protein S19E Chain: BU
30S ribosomal protein S24E Chain: BV
30S ribosomal protein S27E Chain: BW
30S ribosomal protein S28E Chain: BX
30S ribosomal protein S27AE Chain: BY
50S ribosomal protein L14E Chains: A5, AK
50S ribosomal protein L1P Chain: AA
50S ribosomal protein L31E Chain: Aa
50S ribosomal protein L2 Chain: AB
50S ribosomal protein L32E Chain: Ab
50S ribosomal protein L3P Chain: AC
50S ribosomal protein L4P Chain: AD
50S ribosomal protein L34E Chain: Ad
50S ribosomal protein L5P Chain: AE
50S ribosomal protein L37E Chain: Ae
50S ribosomal protein L6P Chain: AF
50S ribosomal protein L40E Chain: Ag
50S ribosomal protein L11P Chain: AH
50S ribosomal protein L41E Chain: Ah
50S ribosomal protein L13P Chain: AI
50S ribosomal protein L37AE Chain: Ai
50S ribosomal protein L14P Chain: AJ
50S ribosomal protein L44E Chain: Aj
50S ribosomal protein P0/L10E Chain: Ak
50S ribosomal protein L15P Chain: AL
50S ribosomal protein L15E Chain: AM
50S ribosomal protein L10E/L16 Chain: AN
50S ribosomal protein L18P Chain: AO
50S ribosomal protein L18E Chain: AP
50S ribosomal protein L21E Chain: AR
50S ribosomal protein L24E Chain: AV
50S ribosomal protein L30P Chain: AY
50S ribosomal protein L30E Chain: AZ
E-tRNA Chain: B1
16S ribosomal RNA Chain: B2
23S ribosomal RNA Chain: A1
5S ribosomal RNA Chain: A3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-5691
Expression system: Escherichia coli BL21(DE3)