4v4w

Electron Microscopy
15Å resolution

Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143

Released:
Source organism: Escherichia coli
Related structures: EMD-1143

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
PDBe Complex ID:
PDB-CPX-135579 (preferred)
Entry contents:
45 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (49 distinct):
Small ribosomal subunit protein uS2 Chain: AB
Molecule details ›
Chain: AB
Length: 236 amino acids
Theoretical weight: 26.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 6-241; Coverage: 98%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: AC
Molecule details ›
Chain: AC
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 2-207; Coverage: 88%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: AD
Small ribosomal subunit protein uS5 Chain: AE
Small ribosomal subunit protein bS6, non-modified isoform Chain: AF
Small ribosomal subunit protein uS7 Chain: AG
Small ribosomal subunit protein uS8 Chain: AH
Small ribosomal subunit protein uS9 Chain: AI
Small ribosomal subunit protein uS10 Chain: AJ
Small ribosomal subunit protein uS11 Chain: AK
Small ribosomal subunit protein uS12 Chain: AL
Small ribosomal subunit protein uS13 Chain: AM
Small ribosomal subunit protein uS14 Chain: AN
Small ribosomal subunit protein uS15 Chain: AO
Small ribosomal subunit protein bS16 Chain: AP
Small ribosomal subunit protein uS17 Chain: AQ
Small ribosomal subunit protein bS18 Chain: AR
Small ribosomal subunit protein uS19 Chain: AS
Small ribosomal subunit protein bS20 Chain: AT
Large ribosomal subunit protein uL1 Chain: B2
Large ribosomal subunit protein bL12 Chains: B3, B5
Large ribosomal subunit protein uL2 Chain: BA
Large ribosomal subunit protein uL3 Chain: BB
Large ribosomal subunit protein uL4 Chain: BC
Large ribosomal subunit protein uL5 Chain: BD
Large ribosomal subunit protein uL6 Chain: BE
Large ribosomal subunit protein bL9 Chain: BF
Large ribosomal subunit protein uL11 Chain: BG
Large ribosomal subunit protein uL13 Chain: BH
Large ribosomal subunit protein uL14 Chain: BI
Large ribosomal subunit protein uL15 Chain: BJ
Large ribosomal subunit protein uL16 Chain: BK
Large ribosomal subunit protein bL17 Chain: BL
Large ribosomal subunit protein uL18 Chain: BM
Large ribosomal subunit protein bL19 Chain: BN
Large ribosomal subunit protein bL20 Chain: BO
Large ribosomal subunit protein uL22 Chain: BQ
Large ribosomal subunit protein uL23 Chain: BR
Large ribosomal subunit protein uL24 Chain: BS
Large ribosomal subunit protein bL25 Chain: BT
Large ribosomal subunit protein bL27 Chain: BU
Large ribosomal subunit protein uL29 Chain: BW
Large ribosomal subunit protein uL30 Chain: BX
Large ribosomal subunit protein bL32 Chain: BZ
Large ribosomal subunit protein bL33 Chain: B1
16S ribosomal RNA Chain: AA
tRNA Chains: AU, AV, AW
23S ribosomal RNA Chain: B0
5S ribosomal RNA Chain: B9

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 15Å
Relevant EMDB volumes: EMD-1143