› 4v9p

X-ray diffraction
2.9Å resolution

Control of ribosomal subunit rotation by elongation factor G

Released:
DOI: 10.2210/pdb4v9p/pdb
Model geometry
Fit model/data
PDB entry 4v9p coloured by chain, front view.
PDB entry 4v9p coloured by chain, side view.
PDB entry 4v9p coloured by chain, top view.
Source organisms:
Primary publication:
Control of ribosomal subunit rotation by elongation factor G.
Pulk A, Cate JH
Science 340 1235970 (2013)
PMID: 23812721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero 55-mer (preferred)
hetero 54-mer
hetero 53-mer
PDBe Complex ID:
PDB-CPX-289468 (preferred)
Entry contents:
52 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein uL2 Chains: AC, CC, EC, GC
Molecule details ›
Chains: AC, CC, EC, GC
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chains: AD, CD, ED, GD
Molecule details ›
Chains: AD, CD, ED, GD
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chains: AE, CE, EE, GE
Large ribosomal subunit protein uL5 Chains: AF, CF, EF, GF
Large ribosomal subunit protein uL6 Chains: AG, CG, EG, GG
Large ribosomal subunit protein bL9 Chains: AH, CH, EH, GH
Large ribosomal subunit protein uL11 Chains: AI, CI, EI, GI
Large ribosomal subunit protein uL13 Chains: AJ, CJ, EJ, GJ
Large ribosomal subunit protein uL14 Chains: AK, CK, EK, GK
Large ribosomal subunit protein uL15 Chains: AL, CL, EL, GL
Large ribosomal subunit protein uL16 Chains: AM, CM, EM, GM
Large ribosomal subunit protein bL17 Chains: AN, CN, EN, GN
Large ribosomal subunit protein uL18 Chains: AO, CO, EO, GO
Large ribosomal subunit protein bL19 Chains: AP, CP, EP, GP
Large ribosomal subunit protein bL20 Chains: AQ, CQ, EQ, GQ
Large ribosomal subunit protein bL21 Chains: AR, CR, ER, GR
Large ribosomal subunit protein uL22 Chains: AS, CS, ES, GS
Large ribosomal subunit protein uL23 Chains: AT, CT, ET, GT
Large ribosomal subunit protein uL24 Chains: AU, CU, EU, GU
Large ribosomal subunit protein bL25 Chains: AV, CV, EV, GV
Large ribosomal subunit protein bL27 Chains: AW, CW, EW, GW
Large ribosomal subunit protein bL28 Chains: AX, CX, EX, GX
Large ribosomal subunit protein uL29 Chains: AY, CY, EY, GY
Large ribosomal subunit protein uL30 Chains: AZ, CZ, EZ, GZ
Large ribosomal subunit protein bL32 Chains: A0, C0, E0, G0
Large ribosomal subunit protein bL33 Chains: A1, C1, E1, G1
Large ribosomal subunit protein bL34 Chains: A2, C2, E2, G2
Large ribosomal subunit protein bL35 Chains: A3, C3, E3, G3
Large ribosomal subunit protein bL36A Chains: A4, C4, E4, G4
Large ribosomal subunit protein uL10 Chains: A5, E5
Small ribosomal subunit protein uS2 Chains: BB, DB, FB, HB
Small ribosomal subunit protein uS3 Chains: BC, DC, FC, HC
Small ribosomal subunit protein uS4 Chains: BD, DD, FD, HD
Small ribosomal subunit protein uS5 Chains: BE, DE, FE, HE
Small ribosomal subunit protein bS6 Chains: BF, DF, FF, HF
Small ribosomal subunit protein uS7 Chains: BG, DG, FG, HG
Small ribosomal subunit protein uS8 Chains: BH, DH, FH, HH
Small ribosomal subunit protein uS9 Chains: BI, DI, FI, HI
Small ribosomal subunit protein uS10 Chains: BJ, DJ, FJ, HJ
Small ribosomal subunit protein uS11 Chains: BK, DK, FK, HK
Small ribosomal subunit protein uS12 Chains: BL, DL, FL, HL
Small ribosomal subunit protein uS13 Chains: BM, DM, FM, HM
Small ribosomal subunit protein uS14 Chains: BN, DN, FN, HN
Small ribosomal subunit protein uS15 Chains: BO, DO, FO, HO
Small ribosomal subunit protein bS16 Chains: BP, DP, FP, HP
Small ribosomal subunit protein uS17 Chains: BQ, DQ, FQ, HQ
Small ribosomal subunit protein bS18 Chains: BR, DR, FR, HR
Small ribosomal subunit protein uS19 Chains: BS, DS, FS, HS
Small ribosomal subunit protein bS20 Chains: BT, DT, FT, HT
Small ribosomal subunit protein bS21 Chains: BU, DU, FU, HU
Elongation factor G Chains: BV, DV, FV, HV
Viomycin Chains: BW, DW, FW
23S ribosomal RNA Chains: AA, CA, EA, GA
5S ribosomal RNA Chains: AB, CB, EB, GB
16S ribosomal RNA Chains: BA, DA, FA, HA

Ligands and Environments

3 bound ligands:
Ligand MG 740 x MG
Ligand ZN 4 x ZN
Ligand GCP 4 x GCP
3 modified residues:
Ligand KBE 3 x KBE
Ligand DPP 3 x DPP
Ligand 5OH 3 x 5OH

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1, ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 361.14Å b: 360.51Å c: 429.73Å
α: 90° β: 103.217° γ: 90°
R-values:
R R work R free
0.215 0.215 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Bacterial Protein Synthesis as a Target for Antibiotic Inhibition.
Arenz et al. (2016)
15 more

12 mentions without citation

How does RNA fold dynamically?
Bushhouse et al. (2022)
11 more