› 4w4i

X-ray diffraction
2.85Å resolution

Crystal structure of EspG3 from the ESX-3 type VII secretion system of M. tuberculosis

Released:
DOI: 10.2210/pdb4w4i/pdb
PDB entry 4w4i coloured by chain, front view.
PDB entry 4w4i coloured by chain, side view.
PDB entry 4w4i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis str. Erdman = ATCC 35801
Primary publication:
Structure of a PE-PPE-EspG complex from Mycobacterium tuberculosis reveals molecular specificity of ESX protein secretion.
Ekiert DC, Cox JS
Proc Natl Acad Sci U S A 111 14758-63 (2014)
PMID: 25275011

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESX-3 secretion-associated protein EspG3 Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 33.62 KDa
Source organism: Mycobacterium tuberculosis str. Erdman = ATCC 35801
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJC6 (Residues: 1-295; Coverage: 100%)
Gene names: MT0302, espG3
Sequence domains: EspG family

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 74.98Å b: 96.31Å c: 94.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.225 0.274
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Bacterial Secretion Systems: An Overview.
Green et al. (2016)
16 more

4 mentions without citation

Bacterial secretion chaperones: the mycobacterial type VII case.
Phan et al. (2018)
3 more