› 4w5k

X-ray diffraction
1.7Å resolution

Structure of a mitochondrial aspartate aminotransferase from Trypanosoma brucei, K237A mutant

Released:
DOI: 10.2210/pdb4w5k/pdb
PDB entry 4w5k coloured by chain, front view.
PDB entry 4w5k coloured by chain, side view.
PDB entry 4w5k coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Primary publication:
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Wall A, Clifton MC, Lukacs CM, Staker BL, Van Voorhis W, Myler P, Lorimer DD, Edwards TE
Acta Crystallogr F Struct Biol Commun 71 566-71 (2015)
PMID: 25945710

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.82 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q385Q9 (Residues: 1-388; Coverage: 100%)
Gene name: Tb11.02.2740
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand MLT 2 x MLT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 62.39Å b: 96.66Å c: 81.61Å
α: 90° β: 111.03° γ: 90°
R-values:
R R work R free
0.154 0.153 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids.
Marchese et al. (2018)
1 more

2 mentions without citation

Structural Insights into a Novel Class of Aspartate Aminotransferase from Corynebacterium glutamicum.
Son et al. (2016)
1 more