› 4w96

X-ray diffraction
1.5Å resolution

Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked with 5mM [Ru(CO)3Cl2]2 followed by the reaction in deoxy-myoglobin solution

Released:
DOI: 10.2210/pdb4w96/pdb
PDB entry 4w96 coloured by chain, front view.
PDB entry 4w96 coloured by chain, side view.
PDB entry 4w96 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Preparation of a cross-linked porous protein crystal containing Ru carbonyl complexes as a CO-releasing extracellular scaffold.
Tabe H, Fujita K, Abe S, Tsujimoto M, Kuchimaru T, Kizaka-Kondoh S, Takano M, Kitagawa S, Ueno T
Inorg Chem 54 215-20 (2015)
PMID: 25494847

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

5 bound ligands:
Ligand DMF 1 x DMF
Ligand CL 8 x CL
Ligand NA 1 x NA
Ligand RU1 1 x RU1
Ligand RU 8 x RU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P43212
Unit cell:
a: 79.011Å b: 79.011Å c: 36.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.227
Expression system: Gallus gallus

Quick links

Citations

2 review citations

Design of a confined environment using protein cages and crystals for the development of biohybrid materials.
Abe et al. (2016)
1 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)