› 4wa8

X-ray diffraction
2.2Å resolution

Methanopyrus Kandleri FEN-1 nuclease

Released:
DOI: 10.2210/pdb4wa8/pdb
PDB entry 4wa8 coloured by chain, front view.
PDB entry 4wa8 coloured by chain, side view.
PDB entry 4wa8 coloured by chain, top view.
Source organism: Methanopyrus kandleri AV19
Primary publication:
Structure and specificity of FEN-1 from Methanopyrus kandleri.
Shah S, Dunten P, Stiteler A, Park CK, Horton NC
Proteins 83 188-94 (2015)
PMID: 25354467

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 41.02 KDa
Source organism: Methanopyrus kandleri AV19
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TXU4 (Residues: 1-348; Coverage: 100%)
Gene names: MK0566, fen
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 55.331Å b: 86.193Å c: 147.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.231
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structure and specificity of FEN-1 from Methanopyrus kandleri.
Shah et al. (2015)