› 4wb7

X-ray diffraction
1.9Å resolution

Crystal structure of a chimeric fusion of human DnaJ (Hsp40) and cAMP-dependent protein kinase A (catalytic alpha subunit)

Released:
DOI: 10.2210/pdb4wb7/pdb
PDB entry 4wb7 coloured by chain, front view.
PDB entry 4wb7 coloured by chain, side view.
PDB entry 4wb7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into mis-regulation of protein kinase A in human tumors.
Cheung J, Ginter C, Cassidy M, Franklin MC, Rudolph MJ, Robine N, Darnell RB, Hendrickson WA
Proc Natl Acad Sci U S A 112 1374-9 (2015)
PMID: 25605907

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148111 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DnaJ homolog subfamily B member 1; cAMP-dependent protein kinase catalytic subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 47.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25685 (Residues: 2-70; Coverage: 20%)
  • Canonical: P17612 (Residues: 16-351; Coverage: 96%)
Gene names: DNAJ1, DNAJB1, HDJ1, HSPF1, PKACA, PRKACA
Sequence domains:
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chains: I, J
Molecule details ›
Chains: I, J
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61925 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand ZN 15 x ZN
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 59.233Å b: 121.155Å c: 173.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.188
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology.
Turnham et al. (2016)
16 more

12 mentions without citation

Structural insights into mis-regulation of protein kinase A in human tumors.
Cheung et al. (2015)
11 more