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Measurement of Acceptor-TΨC Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.

Yamashita S, Martinez A, Tomita K
Structure 23 830-842 (2015)
Related entries: 4wby, 4wbz, 4wc0, 4wc1, 4wc2, 4wc4, 4wc5, 4wc6, 4wc7, 4x0a, 4x0b

Cited: 14 times
EuropePMC logo PMID: 25914059

Abstract

The 3'-terminal CCA (C74C75A76-3') of tRNA is required for protein synthesis. In Aquifex aeolicus, the CCA-3' is synthesized by CC-adding and A-adding enzymes, although in most organisms, CCA is synthesized by a single CCA-adding enzyme. The mechanisms by which the A-adding enzyme adds only A76, but not C74C75, onto tRNA remained elusive. The complex structures of the enzyme with various tRNAs revealed the presence of a single tRNA binding site on the enzyme, with the enzyme measuring the acceptor-TΨC helix length of tRNA. The 3'-C75 of tRNA lacking A76 can reach the active site and the size and shape of the nucleotide binding pocket at the insertion stage are suitable for ATP. The 3'-C74 of tRNA lacking C75A76 cannot reach the active site, although CTP or ATP can bind the active pocket. Thus, the A-adding enzyme adds only A76, but not C74C75, onto tRNA.

Reviews citing this publication (4)

  1. The tRNA Elbow in Structure, Recognition and Evolution. Zhang J, Ferré-D'Amaré AR. Life (Basel) 6 E3 (2016)
  2. Function and Regulation of Human Terminal Uridylyltransferases. Yashiro Y, Tomita K. Front Genet 9 538 (2018)
  3. Trying on tRNA for Size: RNase P and the T-box Riboswitch as Molecular Rulers. Zhang J, Ferré-DAmaré AR. Biomolecules 6 E18 (2016)
  4. Human BCDIN3D Is a Cytoplasmic tRNAHis-Specific 5'-Monophosphate Methyltransferase. Tomita K, Liu Y. Front Genet 9 305 (2018)

Articles citing this publication (10)

  1. Crystal structures of U6 snRNA-specific terminal uridylyltransferase. Yamashita S, Takagi Y, Nagaike T, Tomita K. Nat Commun 8 15788 (2017)
  2. Mechanism of 3'-Matured tRNA Discrimination from 3'-Immature tRNA by Class-II CCA-Adding Enzyme. Yamashita S, Tomita K. Structure 24 918-925 (2016)
  3. Divergent Evolution of Eukaryotic CC- and A-Adding Enzymes. Erber L, Franz P, Betat H, Prohaska S, Mörl M. Int J Mol Sci 21 E462 (2020)
  4. Mechanistic insights into tRNA cleavage by a contact-dependent growth inhibitor protein and translation factors. Wang J, Yashiro Y, Sakaguchi Y, Suzuki T, Tomita K. Nucleic Acids Res 50 4713-4731 (2022)
  5. Phylogeny and Evolution of RNA 3'-Nucleotidyltransferases in Bacteria. Jones GH. J Mol Evol 87 254-270 (2019)
  6. Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA Substrates. Hennig O, Philipp S, Bonin S, Rollet K, Kolberg T, Jühling T, Betat H, Sauter C, Mörl M. Int J Mol Sci 21 E9047 (2020)
  7. Individually double minimum-distance definition of protein-RNA binding residues and application to structure-based prediction. Hu W, Qin L, Li M, Pu X, Guo Y. J Comput Aided Mol Des 32 1363-1373 (2018)
  8. Ligand-dependent tRNA processing by a rationally designed RNase P riboswitch. Ender A, Etzel M, Hammer S, Findeiß S, Stadler P, Mörl M. Nucleic Acids Res 49 1784-1800 (2021)
  9. The Thermus thermophilus DEAD-box protein Hera is a general RNA binding protein and plays a key role in tRNA metabolism. Donsbach P, Yee BA, Sanchez-Hevia D, Berenguer J, Aigner S, Yeo GW, Klostermeier D. RNA 26 1557-1574 (2020)
  10. NMR reveals structural rearrangements associated to substrate insertion in nucleotide-adding enzymes. Mohanty B, Geralt M, Wüthrich K, Serrano P. Protein Sci 25 917-925 (2016)


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