› 4wck

X-ray diffraction
1.4Å resolution

Crystal Structure of apo Cell Shape Determinant protein Csd4 from Helicobacter pylori

Released:
DOI: 10.2210/pdb4wck/pdb
PDB entry 4wck coloured by chain, front view.
PDB entry 4wck coloured by chain, side view.
PDB entry 4wck coloured by chain, top view.
Source organism: Helicobacter pylori G27
Primary publication:
Helical shape of Helicobacter pylori requires an atypical glutamine as a zinc ligand in the carboxypeptidase Csd4.
Chan AC, Blair KM, Liu Y, Frirdich E, Gaynor EC, Tanner ME, Salama NR, Murphy ME
J Biol Chem 290 3622-38 (2015)
PMID: 25505267

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved hypothetical secreted protein Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 50.18 KDa
Source organism: Helicobacter pylori G27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5ZAD9 (Residues: 23-438; Coverage: 95%)
Gene name: HPG27_353
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand IOD 32 x IOD
Ligand API 1 x API
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 53.28Å b: 66.78Å c: 145.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments.
Yang et al. (2016)
3 more

2 mentions without citation

A Bacterial Cell Shape-Determining Inhibitor.
Liu et al. (2016)
1 more