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Structure of Ribosomal Silencing Factor Bound to Mycobacterium tuberculosis Ribosome.

Li X, Sun Q, Jiang C, Yang K, Hung LW, Zhang J, Sacchettini JC
Structure 23 1858-1865 (2015)
Cited: 26 times
EuropePMC logo PMID: 26299947

Abstract

The ribosomal silencing factor RsfS slows cell growth by inhibiting protein synthesis during periods of diminished nutrient availability. The crystal structure of Mycobacterium tuberculosis (Mtb) RsfS, together with the cryo-electron microscopy (EM) structure of the large subunit 50S of Mtb ribosome, reveals how inhibition of protein synthesis by RsfS occurs. RsfS binds to the 50S at L14, which, when occupied, blocks the association of the small subunit 30S. Although Mtb RsfS is a dimer in solution, only a single subunit binds to 50S. The overlap between the dimer interface and the L14 binding interface confirms that the RsfS dimer must first dissociate to a monomer in order to bind to L14. RsfS interacts primarily through electrostatic and hydrogen bonding to L14. The EM structure shows extended rRNA density that it is not found in the Escherichia coli ribosome, the most striking of these being the extended RNA helix of H54a.

Reviews - 4wcw mentioned but not cited (2)

  1. Production of recombinant proteins in Mycobacterium smegmatis for structural and functional studies. Bashiri G, Baker EN. Protein Sci 24 1-10 (2015)
  2. Translational regulation in mycobacteria and its implications for pathogenicity. Sawyer EB, Grabowska AD, Cortes T. Nucleic Acids Res 46 6950-6961 (2018)

Articles - 4wcw mentioned but not cited (2)

  1. Structures of the human mitochondrial ribosome in native states of assembly. Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V. Nat Struct Mol Biol 24 866-869 (2017)
  2. Structure of Ribosomal Silencing Factor Bound to Mycobacterium tuberculosis Ribosome. Li X, Sun Q, Jiang C, Yang K, Hung LW, Zhang J, Sacchettini JC. Structure 23 1858-1865 (2015)


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  1. Ribosome Hibernation. Prossliner T, Skovbo Winther K, Sørensen MA, Gerdes K. Annu Rev Genet 52 321-348 (2018)
  2. Advanced Methods for Studying Structure and Interactions of Macrolide Antibiotics. Jednačak T, Mikulandra I, Novak P. Int J Mol Sci 21 E7799 (2020)
  3. Advances in Key Drug Target Identification and New Drug Development for Tuberculosis. Mi J, Gong W, Wu X. Biomed Res Int 2022 5099312 (2022)
  4. Coping with stress: How bacteria fine-tune protein synthesis and protein transport. Njenga R, Boele J, Öztürk Y, Koch HG. J Biol Chem 299 105163 (2023)

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  1. Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus. Eyal Z, Matzov D, Krupkin M, Wekselman I, Paukner S, Zimmerman E, Rozenberg H, Bashan A, Yonath A. Proc Natl Acad Sci U S A 112 E5805-14 (2015)
  2. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome. Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N. Cell Rep 20 149-160 (2017)
  3. Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis. Yang K, Chang JY, Cui Z, Li X, Meng R, Duan L, Thongchol J, Jakana J, Huwe CM, Sacchettini JC, Zhang J. Nucleic Acids Res 45 10884-10894 (2017)
  4. De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes. Kappel K, Liu S, Larsen KP, Skiniotis G, Puglisi EV, Puglisi JD, Zhou ZH, Zhao R, Das R. Nat Methods 15 947-954 (2018)
  5. Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein. Gerovac M, El Mouali Y, Kuper J, Kisker C, Barquist L, Vogel J. RNA 26 1448-1463 (2020)
  6. MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation. Kim HJ, Barrientos A. Nucleic Acids Res 46 8435-8453 (2018)
  7. Human GTPBP5 (MTG2) fuels mitoribosome large subunit maturation by facilitating 16S rRNA methylation. Maiti P, Antonicka H, Gingras AC, Shoubridge EA, Barrientos A. Nucleic Acids Res 48 7924-7943 (2020)
  8. Structure of the mature kinetoplastids mitoribosome and insights into its large subunit biogenesis. Soufari H, Waltz F, Parrot C, Durrieu-Gaillard S, Bochler A, Kuhn L, Sissler M, Hashem Y. Proc Natl Acad Sci U S A 117 29851-29861 (2020)
  9. Proteomic profiling of the mitochondrial ribosome identifies Atp25 as a composite mitochondrial precursor protein. Woellhaf MW, Sommer F, Schroda M, Herrmann JM. Mol Biol Cell 27 3031-3039 (2016)
  10. Ribosome Dimerization Protects the Small Subunit. Feaga HA, Kopylov M, Kim JK, Jovanovic M, Dworkin J. J Bacteriol 202 e00009-20 (2020)
  11. Letter Cryo-EM structure of Mycobacterium smegmatis ribosome reveals two unidentified ribosomal proteins close to the functional centers. Li Z, Ge X, Zhang Y, Zheng L, Sanyal S, Gao N. Protein Cell 9 384-388 (2018)
  12. Mechanism of ribosome shutdown by RsfS in Staphylococcus aureus revealed by integrative structural biology approach. Khusainov I, Fatkhullin B, Pellegrino S, Bikmullin A, Liu WT, Gabdulkhakov A, Shebel AA, Golubev A, Zeyer D, Trachtmann N, Sprenger GA, Validov S, Usachev K, Yusupova G, Yusupov M. Nat Commun 11 1656 (2020)
  13. Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases. Lopez Quezada L, Smith R, Lupoli TJ, Edoo Z, Li X, Gold B, Roberts J, Ling Y, Park SW, Nguyen Q, Schoenen FJ, Li K, Hugonnet JE, Arthur M, Sacchettini JC, Nathan C, Aubé J. Front Microbiol 11 1248 (2020)
  14. Identification of Mycobacterial Ribosomal Proteins as Targets for CD4+ T Cells That Enhance Protective Immunity in Tuberculosis. Kennedy SC, Johnson AJ, Bharrhan S, Lindestam Arlehamn CS, Xu J, Garforth SJ, Chan J, Jacobs WR, Sette A, Almo SC, Porcelli SA. Infect Immun 86 e00009-18 (2018)
  15. Replacement of S14 Protein in Ribosomes of Zinc-Starved Mycobacteria Reduces Spectinamide Sensitivity. Li Y, Koripella RK, Sharma MR, Lee RE, Agrawal RK, Ojha AK. Antimicrob Agents Chemother 65 e01833-20 (2021)
  16. HflX is a GTPase that controls hypoxia-induced replication arrest in slow-growing mycobacteria. Ngan JYG, Pasunooti S, Tse W, Meng W, Ngan SFC, Jia H, Lin JQ, Ng SW, Jaafa MT, Cho SLS, Lim J, Koh HQV, Abdul Ghani N, Pethe K, Sze SK, Lescar J, Alonso S. Proc Natl Acad Sci U S A 118 e2006717118 (2021)
  17. Translation of a Leaderless Reporter Is Robust During Exponential Growth and Well Sustained During Stress Conditions in Mycobacterium tuberculosis. Grabowska AD, Andreu N, Cortes T. Front Microbiol 12 746320 (2021)
  18. Y98 Mutation Leads to the Loss of RsfS Anti-Association Activity in Staphylococcus aureus. Fatkhullin B, Golubev A, Garaeva N, Validov S, Gabdulkhakov A, Yusupov M. Int J Mol Sci 23 10931 (2022)


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