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Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.

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Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H
OpenAccess logo Vet Res 46 14 (2015)
Related entries: 4wem, 4weu

Cited: 11 times
EuropePMC logo PMID: 25828907

Abstract

Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding.

Reviews - 4wen mentioned but not cited (1)

  1. Single Domain Antibody application in bacterial infection diagnosis and neutralization. Qin Q, Liu H, He W, Guo Y, Zhang J, She J, Zheng F, Zhang S, Muyldermans S, Wen Y. Front Immunol 13 1014377 (2022)

Articles - 4wen mentioned but not cited (2)

  1. Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies. Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H. Vet Res 46 14 (2015)
  2. Insights into Comparative Modeling of VHH Domains. Vattekatte AM, Cadet F, Gelly JC, de Brevern AG. Int J Mol Sci 22 9771 (2021)


Reviews citing this publication (1)

  1. Evolution and structural dynamics of bacterial glycan binding adhesins. Moonens K, Remaut H. Curr Opin Struct Biol 44 48-58 (2017)

Articles citing this publication (7)

  1. Oral Administration of a Select Mixture of Bacillus Probiotics Affects the Gut Microbiota and Goblet Cell Function following Escherichia coli Challenge in Newly Weaned Pigs of Genotype MUC4 That Are Supposed To Be Enterotoxigenic E. coli F4ab/ac Receptor Negative. Zhang W, Zhu YH, Zhou D, Wu Q, Song D, Dicksved J, Wang JF. Appl Environ Microbiol 83 e02747-16 (2017)
  2. Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model. Virdi V, Palaci J, Laukens B, Ryckaert S, Cox E, Vanderbeke E, Depicker A, Callewaert N. Nat Biotechnol 37 527-530 (2019)
  3. Effects of a microencapsulated formula of organic acids and essential oils on nutrient absorption, immunity, gut barrier function, and abundance of enterotoxigenic Escherichia coli F4 in weaned piglets challenged with E. coli F4. Choi J, Wang L, Liu S, Lu P, Zhao X, Liu H, Lahaye L, Santin E, Liu S, Nyachoti M, Yang C. J Anim Sci 98 skaa259 (2020)
  4. Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization. Vanmarsenille C, Díaz Del Olmo I, Elseviers J, Hassanzadeh Ghassabeh G, Moonens K, Vertommen D, Martel A, Haesebrouck F, Pasmans F, Hernalsteens JP, De Greve H. Vet Res 48 86 (2017)
  5. Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants. Palaci J, Virdi V, Depicker A. Plant Biotechnol J 17 1760-1769 (2019)
  6. Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary. Salhi I, Bessalah S, Snoun D, Khorchani T, Hammadi M. Iran J Biotechnol 18 e2247 (2020)
  7. Development of Antibody-Fragment-Producing Rice for Neutralization of Human Norovirus. Sasou A, Yuki Y, Kurokawa S, Sato S, Goda Y, Uchida M, Matsumoto N, Sagara H, Watanabe Y, Kuroda M, Sakon N, Sugiura K, Nakahashi-Ouchida R, Ushijima H, Fujihashi K, Kiyono H. Front Plant Sci 12 639953 (2021)


Related citations provided by authors (1)

  1. Orally fed seeds producing designer IgAs protect weaned piglets against enterotoxigenic Escherichia coli infection.. Virdi V, Coddens A, De Buck S, Millet S, Goddeeris BM, Cox E, De Greve H, Depicker A Proc Natl Acad Sci U S A 110 11809-14 (2013)

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