› 4wh5

X-ray diffraction
1.82Å resolution

Crystal structure of lincosamide antibiotic adenylyltransferase LnuA, lincomycin-bound

Released:
DOI: 10.2210/pdb4wh5/pdb
PDB entry 4wh5 coloured by chain, front view.
PDB entry 4wh5 coloured by chain, side view.
PDB entry 4wh5 coloured by chain, top view.
Source organism: Staphylococcus haemolyticus
Entry authors: Stogios PJ, Dong A, Minasov G, Evdokimova E, Egorova O, Kudritska M, Yim O, Courvalin P, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lincosamide resistance protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 19.31 KDa
Source organism: Staphylococcus haemolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06107 (Residues: 2-161; Coverage: 99%)
Gene name: linA
Sequence domains: Aminoglycoside-2''-adenylyltransferase
Structure domains: Beta Polymerase; domain 2

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand MG 4 x MG
Ligand 3QB 2 x 3QB
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 56.447Å b: 63.444Å c: 60.808Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.19 0.188 0.23
Expression system: Escherichia coli BL21(DE3)

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