› 4wiq

X-ray diffraction
1.59Å resolution

The structure of Murine alpha-Dystroglycan T190M mutant N-terminal domain.

Released:
DOI: 10.2210/pdb4wiq/pdb
PDB entry 4wiq coloured by chain, front view.
PDB entry 4wiq coloured by chain, side view.
PDB entry 4wiq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The Structure of the T190M Mutant of Murine α-Dystroglycan at High Resolution: Insight into the Molecular Basis of a Primary Dystroglycanopathy.
Bozzi M, Cassetta A, Covaceuszach S, Bigotti MG, Bannister S, Hübner W, Sciandra F, Lamba D, Brancaccio A
OpenAccess logo PLoS One 10 e0124277 (2015)
PMID: 25932631

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-dystroglycan Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 28.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62165 (Residues: 50-313; Coverage: 31%)
Gene name: Dag1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 71.879Å b: 71.879Å c: 144.296Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.146 0.163
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A molecular overview of the primary dystroglycanopathies.
Brancaccio A. (2019)
8 other articles

3 mentions without citation

Expansion of divergent SEA domains in cell surface proteins and nucleoporin 54.
Pei et al. (2017)
2 more